case study at a large university hospital laboratory in Belgium demonstrates the need for clarification on the degrees of freedom laboratories


systemsvirology

The brand new IVD Regulation 2017/746: a case examine at a big college hospital laboratory in Belgium demonstrates the necessity for clarification on the levels of freedom laboratories have to make use of lab-developed assessments to enhance affected person care

Aims The brand new European In Vitro Diagnostic (IVD) Regulation 2017/746 (IVDR) restricts using lab-developed assessments (LDT) after 26th Could 2022. There aren’t any information on the influence of the IVDR on laboratories within the European Union. Strategies Laboratory assessments carried out in UZ Leuven had been divided in 4 teams: core laboratory, immunology, particular chemistry, and molecular microbiology testing.

Every check was labeled as Conformité Européenne (CE)-IVD, modified/off-label CE-IVD, industrial Analysis Use Solely (RUO) or LDT. Every matrix was thought-about a separate check. Outcomes We discovered that 97.6% of the greater than 11.5 million outcomes/yr had been generated with a CE-IVD technique.

Of the 922 completely different laboratory assessments, nevertheless, solely 41.8% had been CE-IVD, 10.8% modified/off-label CE-IVD, 0.3% RUO, and 47.1% LDT. Off-label CE-IVD was primarily used to check various matrices not coated by the declare of the producer (e.g., pleural or peritoneal fluid). LDTs had been primarily used for particular chemistry, move cytometry, and molecular testing.

Excluding move cytometry, the primary causes for the use of 377 LDTs had been lack of a CE-IVD technique (71.9%), analytical necessities (14.3%), and the very fact the LDT was in use earlier than CE-IVD out there (11.9%). Conclusions Whereas the massive majority of outcomes (97.6%) had been generated with a CE-IVD technique, solely 41.8% of laboratory assessments had been CE-IVD.

There may be at present no various in the marketplace for 71.5% of the 537 LDTs carried out in our laboratory which don’t fall throughout the scope of the present IVD directive (IVDD). Compliance with the IVDR would require a significant funding of effort and time.

In vitro diagnostics for screening the blood provide: the brand new European regulation for IVD and the WHO IVD prequalification programme

 

Blood transfusion stays a routine life-saving medical process that helps exchange blood misplaced attributable to surgical procedure, harm or illness. The standard of transfused blood is essential on this course of as blood donors have to be freed from transfusion-transmissible infections and donated blood ought to be suitable to that of the recipient. The standard of donated blood may very well be affected by the standard of in vitro diagnostic medical gadgets (IVDs) used within the screening course of.

Consequently, the necessity for high-quality, protected and well-performing IVDs to be used in transfusion drugs arises, accompanied by the necessity for tight laws on this area. Within the European Union, the brand new IVD Regulation will exchange the prevailing IVD Directive inside a five-year transitional interval. Producers of IVDs are anticipated to completely adjust to the brand new Regulation by 26 Could 2022.

On this assessment, we tackle the key variations regarding advertising and marketing authorization and testing between this new Regulation and its predecessor. We additional current the primary components of the prequalification evaluation launched by the WHO for IVDs, together with disease-specific IVDs for blood screening laboratories.

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Neuron Specific Enolase (NSE)

GWB-50534F 0.1 mg Ask for price

Neuron Specific Enolase (NSE)

CH22126 100 ul of serum
EUR 490.8

Neuron Specific Enolase (NSE)

GWB-87922F 0.1 mg Ask for price

Human Neuron Specific Enolase ELISA kit

E01A6166 96T
EUR 700
Description: ELISA

Human Neuron Specific Enolase ELISA kit

E01N0025-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Neuron Specific Enolase ELISA kit

E01N0025-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Neuron Specific Enolase ELISA kit

E01N0025-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Neuron Specific Enolase (NSE) ELISA

LF-EK0169 1×96T
EUR 723.6

Neuron Specific Enolase Protein

abx060902-100ug 100 ug
EUR 1387.2

Neuron Specific Enolase Protein

abx060902-100g 100 µg Ask for price

Neuron Specific Enolase Protein

abx060902-10g 10 µg
EUR 1862.5

Neuron Specific Enolase Protein

abx060902-50g 50 µg Ask for price

Neuron Specific Enolase Antibody

F48200-0.4ML 0.4 ml
EUR 322.15
Description: NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha to gamma enolase occurs in neural tissue during development in rats and primates. [Wiki]

Neuron Specific Enolase Antibody

GWB-20CCC7 1 mg Ask for price

Neuron Specific Enolase, Antibody

GWB-2648D8 1 ml Ask for price

Neuron Specific Enolase Antibody

GWB-278967 0.2 mg Ask for price

Neuron Specific Enolase Antibody

GWB-5A16BD 0.2 mg Ask for price

Neuron Specific Enolase Antibody

GWB-5D7D7D 0.1 ml Ask for price

Neuron Specific Enolase Antibody

GWB-39ACE9 1 mg Ask for price

Neuron Specific Enolase Antibody

GWB-1C6640 1 mg Ask for price

Neuron Specific Enolase, Antibody

GWB-B690B7 7 ml Ask for price

Neuron Specific Enolase Antibody

GWB-7A81C1 1 mg Ask for price

Neuron Specific Enolase Antibody

GWB-931EEF 1 mg Ask for price

Neuron Specific Enolase Antibody

GWB-6DF4F0 0.2 mg Ask for price

Neuron Specific Enolase Antibody

GWB-BAA2AA 1 mg Ask for price

Neuron Specific Enolase Antibody

GWB-D41123 1 ml Ask for price

Human Enolase Neuron Specific ELISA Kit

IHUNSEKT each
EUR 667
Description: Human Enolase Neuron Specific ELISA Kit

Neuron Specific Enolase 2 Human Recombinant

rAP-1076 Inquiry Ask for price

Neuron Specific Enolase (NSE) 98%

GWB-104D9E 1 mg Ask for price

Neuron Specific Enolase (NSE)/ENO2

CH23003 100 ul
EUR 214.8

Neuron Specific Enolase (NSE)/ENO2

RA22110 100 ul
EUR 490.8

Human Enolase, Neuron Specific (ENO2) CLIA Kit

20-abx491609
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human NSE (Neuron Specific Enolase) ELISA Kit

EKE60659-5x96T 5x96T
EUR 2667.6

Human NSE (Neuron Specific Enolase) ELISA Kit

EKE60659-96T 96T
EUR 561.6

Human NSE(Neuron Specific Enolase) ELISA Kit

RE3008H-48wells 48 wells
EUR 116.55

Human NSE(Neuron Specific Enolase) ELISA Kit

RE3008H-96wells 96 wells
EUR 161.55

Human Enolase, Neuron Specific (NSE) Protein

20-abx066423
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  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Enolase, Neuron Specific (NSE) Protein

20-abx066426
  • Ask for price
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  • Ask for price
  • Ask for price
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Human Enolase, Neuron Specific (NSE) Protein

abx066423-100g 100 µg
EUR 475

Human Enolase, Neuron Specific (NSE) Protein

abx066423-10g 10 µg
EUR 200

Human Enolase, Neuron Specific (NSE) Protein

abx066423-50g 50 µg
EUR 337.5

Human Enolase, Neuron Specific (NSE) Protein

abx066426-100g 100 µg
EUR 475

Human Enolase, Neuron Specific (NSE) Protein

abx066426-10g 10 µg
EUR 200

Human Enolase, Neuron Specific (NSE) Protein

abx066426-50g 50 µg
EUR 337.5

Human Neuron Specific Enolase (NSE) ELISA (4X96T)

LF-EK0170 4×96T
EUR 2454

Human Enolase, Neuron Specific (ENO2) ELISA Kit

abx573286-96tests 96 tests
EUR 877.2

Human Enolase, Neuron Specific (ENO2) ELISA Kit

abx253091-96tests 96 tests
EUR 877.2

Human Enolase, Neuron Specific (ENO2) ELISA Kit

20-abx152555
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  • 10 × 96 tests
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  • 96 tests

Neuron Specific Enolase (NSE) from Human Brain

GWB-7C3F42 1 mg Ask for price

Neuron Specific Enolase ELISA Kit

E4703-100 each
EUR 705.6

Human Neuron Specific Enolase (NSE) ELISA (10X96T)

LF-EK0171 10×96T
EUR 5380.8

Human Neuron Specific Enolase (NSE) ELISA (20X96T)

LF-EK0172 20×96T
EUR 9369.6

Neuron Specific Enolase Antibody (NSE)

F48200-0.08ML 0.08 ml
EUR 140.25
Description: NSE is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha to gamma enolase occurs in neural tissue during development in rats and primates. [Wiki]

Neuron Specific Enolase Antibody (NSE)

F48201-0.08ML 0.08 ml
EUR 140.25
Description: Neuron Specific Enolase, also called Gamma-enolase, and Enolase 2 (ENO2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt]

Neuron Specific Enolase (NSE) Antibody

E63C00701a 100μg/100μl
EUR 225
Description: Available in various conjugation types.

NSE Antibody / Neuron Specific Enolase

F54038-0.1ML 0.1 ml
EUR 322.15
Description: Neuron Specific Enolase (NSE, Enolase-2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

NSE Antibody / Neuron Specific Enolase

F54039-0.1ML 0.1 ml
EUR 322.15
Description: Neuron Specific Enolase (NSE, Enolase-2) has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Neuron Specific Enolase (NSE), Antibody

GWB-2ABB27 1 mg Ask for price

Neuron Specific Enolase (NSE), Antibody

GWB-1DB347 1 mg Ask for price

Neuron Specific Enolase (NSE) Antibody

abx020636-1mg 1 mg
EUR 994.8

Neuron Specific Enolase (NSE) Antibody

abx020637-1mg 1 mg
EUR 994.8

Neuron Specific Enolase (NSE) Antibody

abx020638-1mg 1 mg
EUR 994.8

Neuron Specific Enolase (NSE) Antibody

abx020639-1mg 1 mg
EUR 994.8

Neuron Specific Enolase (NSE) Antibody

abx020640-1mg 1 mg
EUR 994.8

Neuron Specific Enolase (NSE) Antibody

abx020641-1mg 1 mg
EUR 994.8

Neuron Specific Enolase (NSE), Antibody

GWB-365C9F 1 mg Ask for price

NSE Antibody / Neuron Specific Enolase

RQ4566 100ug
EUR 356.15
Description: NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

NSE Antibody / Neuron Specific Enolase

RQ5267 100ul
EUR 356.15
Description: The ENO2 gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [RefSeq]

Neuron Specific Enolase (NSE), Antibody

GWB-CA6A00 1 mg Ask for price

Neuron Specific Enolase (NSE), Antibody

GWB-C37544 1 mg Ask for price

NSE Antibody / Neuron Specific Enolase

V7210-100UG 100 ug
EUR 424.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V7210-20UG 20 ug
EUR 186.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V7210SAF-100UG 100 ug
EUR 424.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-enolase/neuron specific enolase/enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V7731-100UG 100 ug
EUR 424.15
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V7731-20UG 20 ug
EUR 186.15
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V7731SAF-100UG 100 ug
EUR 424.15
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V8361-100UG 100 ug
EUR 424.15
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V8361-20UG 20 ug
EUR 186.15
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V8361SAF-100UG 100 ug
EUR 424.15
Description: This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3398-100UG 100 ug
EUR 424.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3398-20UG 20 ug
EUR 186.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3398SAF-100UG 100 ug
EUR 424.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3400-100UG 100 ug
EUR 424.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3400-20UG 20 ug
EUR 186.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3400SAF-100UG 100 ug
EUR 424.15
Description: Recognizes a protein of about 50kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3498-100UG 100 ug
EUR 424.15
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3498-20UG 20 ug
EUR 186.15
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3498IHC-7ML 7 ml
EUR 424.15
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

NSE Antibody / Neuron Specific Enolase

V3498SAF-100UG 100 ug
EUR 424.15
Description: This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Neuron Specific Enolase (NSE) Antibody

abx020636-400l 400 µl Ask for price

Neuron Specific Enolase (NSE) Antibody

abx020636-80l 80 µl
EUR 887.5

Neuron Specific Enolase (NSE) Antibody

abx020637-100l 100 µl
EUR 712.5

Neuron Specific Enolase (NSE) Antibody

abx020639-400l 400 µl Ask for price

Neuron Specific Enolase (NSE) Antibody

abx020639-80l 80 µl
EUR 912.5

Neuron Specific Enolase (NSE) Antibody

abx020640-400l 400 µl Ask for price

Neuron Specific Enolase (NSE) Antibody

abx020640-80l 80 µl
EUR 912.5

Neuron Specific Enolase (NSE) Antibody

abx020641-100l 100 µl
EUR 912.5

Human Enolase, Neuron Specific (NSE) ELISA Kit

DLR-NSE-Hu 96T
EUR 422
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

DLR-NSE-Hu-48T 48T
EUR 574.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Enolase, Neuron Specific (NSE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

DLR-NSE-Hu-96T 96T
EUR 745.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Enolase, Neuron Specific (NSE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human NSE(Enolase, Neuron Specific) ELISA Kit

ELK2587-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NSE in the samples is then determined by comparing the OD of the samples to the standard curve.

Human NSE(Enolase, Neuron Specific) ELISA Kit

ELK2587-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NSE in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Enolase, Neuron Specific (NSE) ELISA Kit

EKU03903-48T 48T
EUR 500.5

Human Enolase, Neuron Specific (NSE) ELISA Kit

EKU03903-5x96T 5x96T
EUR 3396.25

Human Enolase, Neuron Specific (NSE) ELISA Kit

EKU03903-96T 96T
EUR 715

Human Enolase, Neuron Specific (NSE) ELISA Kit

EKN45014-48T 48T
EUR 341.04

Human Enolase, Neuron Specific (NSE) ELISA Kit

EKN45014-5x96T 5x96T
EUR 2314.2

Human Enolase, Neuron Specific (NSE) ELISA Kit

EKN45014-96T 96T
EUR 487.2

Human Enolase, Neuron Specific (NSE) ELISA Kit

DL-NSE-Hu 96T
EUR 402
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

EK15827 96Т
EUR 768

Human Enolase, Neuron Specific (NSE) ELISA Kit

RD-NSE-Hu-48T 48T
EUR 411.8
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

RD-NSE-Hu-48Tests 48 Tests
EUR 573.6

Human Enolase, Neuron Specific (NSE) ELISA Kit

RD-NSE-Hu-96T 96T
EUR 588.3
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

RD-NSE-Hu-96Tests 96 Tests
EUR 794.4

Human Enolase, Neuron Specific ELISA Kit (NSE)

RK01966 96 Tests
EUR 280

Human Enolase, Neuron Specific (NSE) ELISA Kit

RDR-NSE-Hu-48T 48T
EUR 432.39
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

RDR-NSE-Hu-48Tests 48 Tests
EUR 600

Human Enolase, Neuron Specific (NSE) ELISA Kit

RDR-NSE-Hu-96T 96T
EUR 617.72
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

RDR-NSE-Hu-96Tests 96 Tests
EUR 830.4

Human Enolase, Neuron Specific (NSE) ELISA Kit

SEA537Hu-10x96wellstestplate 10x96-wells test plate
EUR 5128.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

SEA537Hu-1x48wellstestplate 1x48-wells test plate
EUR 527.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

SEA537Hu-1x96wellstestplate 1x96-wells test plate
EUR 702.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

SEA537Hu-5x96wellstestplate 5x96-wells test plate
EUR 2799.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Enolase, Neuron Specific (NSE) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Enolase, Neuron Specific (NSE) ELISA Kit

4-SEA537Hu
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  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Enolase, Neuron Specific (NSE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Enolase, Neuron Specific (ENO2) Antibody

abx036140-100ug 100 ug
EUR 469.2

Enolase, Neuron Specific (ENO2) Antibody

20-abx112301
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  • 150 ul
  • 50 ul

Enolase, Neuron Specific (ENO2) Antibody

20-abx109806
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Enolase, Neuron Specific (ENO2) Antibody

20-abx109807
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ELISA kit for Human NSE (Neuron Specific Enolase)

E-EL-H1047 1 plate of 96 wells
EUR 452.4
Description: A sandwich ELISA kit for quantitative measurement of Human NSE (Neuron Specific Enolase) in samples from Serum, Plasma, Cell supernatant

Rat Neuron Specific Enolase ELISA kit

E02N0025-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Neuron Specific Enolase ELISA kit

E02N0025-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Neuron Specific Enolase ELISA kit

E02N0025-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Neuron Specific Enolase ELISA kit

E08N0025-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Neuron Specific Enolase ELISA kit

E08N0025-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Neuron Specific Enolase ELISA kit

E08N0025-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Neuron Specific Enolase ELISA kit

E07N0025-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Neuron Specific Enolase ELISA kit

E07N0025-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Neuron Specific Enolase ELISA kit

E07N0025-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Neuron Specific Enolase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Neuron Specific Enolase ELISA kit

E01A14913 96T
EUR 700
Description: ELISA

Neuron Specific Enolase (NSE) 98% Bovine

GWB-677637 0.1 mg Ask for price

Neuron Specific Enolase (NSE) (clone 5E2)

MM-1024-01 0.5 ml
EUR 175

Neuron Specific Enolase (NSE) (clone 5E2)

MM-1024-02 1.0 ml
EUR 254.8

Enolase, Neuron Specific (NSE) Antibody

20-abx270247
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  • 100 tests
  • 200 tests
  • 25 tests
  • 500 tests
  • 50 tests

Enolase, Neuron Specific (NSE) Antibody

20-abx129524
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Enolase, Neuron Specific (NSE) Antibody

20-abx172246
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  • 1 mg
  • 200 ug

Enolase, Neuron Specific (NSE) Antibody

20-abx172247
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  • 1 mg
  • 200 ug

Enolase, Neuron Specific (NSE) Antibody

20-abx102347
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Enolase, Neuron Specific (NSE) Antibody

20-abx102348
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Enolase, Neuron Specific (NSE) Antibody

20-abx102349
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Enolase, Neuron Specific (NSE) Antibody

20-abx102350
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Enolase, Neuron Specific (NSE) Antibody

abx270247-1ml 1 ml
EUR 387.5

Enolase, Neuron Specific (NSE) Antibody

abx270247-200l 200 µl
EUR 275

Enolase, Neuron Specific (NSE) Antibody

abx129524-100l 100 µl
EUR 262.5

Enolase, Neuron Specific (NSE) Antibody

abx129524-1ml 1 ml
EUR 687.5

Enolase, Neuron Specific (NSE) Antibody

abx129524-200l 200 µl
EUR 325

Enolase, Neuron Specific (NSE) Antibody

abx102347-100l 100 µl
EUR 250

Enolase, Neuron Specific (NSE) Antibody

abx102347-1ml 1 ml
EUR 675

Enolase, Neuron Specific (NSE) Antibody

abx102347-200l 200 µl
EUR 312.5

Enolase, Neuron Specific (NSE) Antibody

abx102348-100l 100 µl
EUR 250

Enolase, Neuron Specific (NSE) Antibody

abx102348-1ml 1 ml
EUR 675

Enolase, Neuron Specific (NSE) Antibody

abx102348-200l 200 µl
EUR 312.5

Enolase, Neuron Specific (NSE) Antibody

abx102349-100l 100 µl
EUR 262.5

Enolase, Neuron Specific (NSE) Antibody

abx102349-1ml 1 ml
EUR 687.5

Enolase, Neuron Specific (NSE) Antibody

abx102349-200l 200 µl
EUR 325

Enolase, Neuron Specific (NSE) Antibody

abx102350-100l 100 µl
EUR 262.5

Simultaneous detection of a number of mRNAs and proteins in bovine IVD cells and tissue with single cell decision

 

Aims: Interactions of cells with their neighbors and influences by the encircling extracellular matrix (ECM) is mirrored in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells relying on ECM signalling cues akin to these of the intervertebral disc (IVD), this info is obscured or misplaced when cells are pooled for the generally used transcript evaluation by quantitative PCR or RNA sequencing.

As a substitute, these cells require means to analyse RNA transcript and protein distribution at a single cell or subcellular degree to establish completely different cell sorts and features, with out eradicating them from their surrounding signalling cues.

Outcomes: We developed a easy, sequential protocol combining RNA is situ hybridisation (RISH) and immunohistochemistry (IHC) for the simultaneous evaluation of a number of transcripts alongside proteins. This permits one to characterize heterogeneous cell populations on the single cell degree within the pure cell surroundings and signalling context, each in vivo and in vitro. This protocol is demonstrated on cells of the bovine IVD, for transcripts and proteins concerned in mechanotransduction, stemness and cell proliferation.

Conclusions: A easy, sequential protocol combining RISH and IHC is introduced that enables for simultaneous info on RNA transcripts and proteins to characterize cells inside a heterogeneous cell inhabitants and complicated signalling environments akin to these of the IVD.

Present standing of improvement of methylation biomarkers for in vitro diagnostic IVD functions

 

A big quantity of analysis clearly exhibits that disease-related methylation adjustments can be utilized as biomarkers in any respect phases of scientific illness administration, together with danger evaluation and predisposition screening by way of early diagnostics to personalization of affected person care and monitoring of the relapse and power illness. Thus disease-related methylation adjustments are a pretty supply of the biomarkers that may have important influence on precision drugs. Nevertheless, the interpretation of the analysis findings in methylation biomarkers subject to scientific observe is on the very least not passable. That’s primarily as a result of the proof generated in analysis research indicating the utility of the disease-related methylation change to predict scientific final result is in majority of the instances not enough to postulate the diagnostic use of the biomarker.

The analysis research must be adopted by well-designed and systematic investigations of scientific utility of the biomarker that produce information of enough high quality to satisfy regulatory approval for the check for use to make clinically legitimate choice. On this assessment, we describe methylation-based IVD assessments at present permitted for IVD use or on the superior phases of the event for the diagnostic use. For every of these assessments, we analyze the applied sciences that the check makes use of for methylation detection in addition to describe the sorts of the scientific research that had been carried out to indicate scientific validity of the check and warrant regulatory approval. The examples reviewed right here ought to assist with planning of scientific investigations and supply of the scientific proof required for the regulatory approval of potential methylation biomarker primarily based IVD assessments.

 

Lack of sensitivity of an IVD/CE-labeled package focusing on the S gene for detection of SARS-CoV-2

Aims: New molecular assessments for SARS-CoV-2 are quickly launched in response to the COVID-19 pandemic. The goal of this examine was to guage the analytical and the scientific efficiency of the VIASURE SARS-CoV-2 S gene RT-PCR Equipment on the BD Max™ system and to match outcomes with these obtained with the cobas® SARS-CoV-2 check on the cobas® 6800 system.

Strategies: For testing the analytical efficiency, reference materials was used. Scientific samples (n=101) obtained from sufferers with signs suitable to COVID-19 had been studied. Oro- and nasopharyngeal swabs had been collected by utilizing both ESwab™ or UTM™ assortment techniques.

Outcomes: When the analytical efficiency was evaluated, the pattern containing the bottom SARS-CoV-2 focus examined damaging with the VIASURE check whereas outcomes obtained with the cobas® check had been discovered to be concordant with the outcomes anticipated. Six out of the 101 scientific samples (5.9%) confirmed an inhibition with the VIASURE check. When analyzing the remaining 95 scientific samples, 27 had been discovered to be damaging with each assays. Of 68 samples optimistic with the cobas® check, the VIASURE check missed 21 (30.9 %) samples. All of these 21 samples had proven Ct values ≥ 31 with the cobas® 6800 system. Not one of the samples examined optimistic with the VIASURE check and damaging with the cobas® check.

Conclusions: The VIASURE check was impaired by an absence of sensitivity and a comparatively excessive variety of invalid outcomes. When utilizing the VIASURE check for routine testing, a major variety of COVID-19 optimistic samples would have been missed.

 

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