Purpose: To obtain samples for transcriptional analysis and proteomics in triplicate from Calu-3 cells infected with A/VN/1203/04 H5N1virus
Overview of Experiment: Time Points = 0, 3, 7, 12, 18, and 24 hrs post infection. Done in triplicate for both RNA and Protein Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates. Time matched mocks done in triplicate from same cell stock as rest of samples. Culture medium (the same as what the virus stock is in) will be used for the mock infections.
Inadvertently, samples for plaque assays were not collected for the 0 and 3 h time points in this experiment. This graph has data from this experiment (ICL004) and a comparable earlier experiment (ICL002) in which we have viral titers for the earlier time points. For both experiments the MOI was 1.0. Attached it the excel file containing these data.