Overview 

Purpose:
To obtain samples for transcriptional analysis and proteomics in triplicate from Calu-3 cell infected with A/CA/04/2009 H1N1 virus.

Overview of Experiment:
Time Points = 0, 3, 7, 12, 18, 24, 30, 36, and 48 hrs post infection.
Done in triplicate for both RNA and Protein
Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates.
Time matched mocks done in triplicate from same cell stock as rest of samples.
Culture medium (the same as what the virus stock is in) will be used for the mock infections.

Infection was be done at an MOI of 3.

In addition to microarray analysis and global proteomics, we have the following metadata.

  • Viral titers derived from plaque assays
  • Relative amounts of viral RNA (qPCR assays)
  • Cytopathic effects (CPE)
These data are available below.

 


Viral Growth Curve 

Attached it the excel file containing these data.

  Attached Files  
   
 ICL006 Growth Curve.xls
 image002.png


Viral RNA 

Attached is the Excel file containing these data. (See time point average worksheet.)

  Attached Files  
   
 ICL006_viral qPCR_analysis.xlsx
 image007.png


CPE and percent infected cells 

 

0hr

3hr

7hr

12hr

18hr

24hr

30hr

36hr

48hr

CPE

-

-

-

+/-

+

+

++

++/+++

+++/++++

~% of cells off of plate

0

0

0

0

0

0

10%

30%

50%

% infected cell

0

0

47

54

72

79

81

87

92

 


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