To obtain samples for transcriptional analysis and proteomics in triplicate from Calu-3 cell infected with A/CA/04/2009 H1N1 virus.
Overview of Experiment:
Time Points = 0, 3, 7, 12, 18, 24, 30, 36, and 48 hrs post infection.
Done in triplicate for both RNA and Protein
Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates.
Time matched mocks done in triplicate from same cell stock as rest of samples.
Culture medium (the same as what the virus stock is in) will be used for the mock infections.
Infection was be done at an MOI of 3.
In addition to microarray analysis and global proteomics, we have the following metadata.
These data are available below.
Viral titers derived from plaque assays
Relative amounts of viral RNA (qPCR assays)
Cytopathic effects (CPE)