Overview 

Purpose: To compare the host response between A/VN/1203/04 H5N1 to the VN1203 HA avirulent mutant virus.

General Design:

20 week-old C57BL/6 mice
VN1203 HA avirulent
Three Doses = 10^3, 10^4, 10^5 PFU
Time points of 1, 2, 4 and 7 days.
5 mice/time point for infections
3 mice/timepoint for time matched mocks
Lung tissue for only the10^4 dose was processed for microarray analysis and global proteomics. However, we have the following metadata for all doses.
  • Animal survival
  • Animal weight loss
  • Viral load in lung tissue derived from plaque assays
  • Relative amounts of viral RNA (qPCR assays)
These are available below.


Animal Survival 

 

10^3 PFU

10^4 PFU

10^5 PFU

Day P.I.

% survival

% survival

% survival

1

100

100

100

2

100

100

100

3

100

100

100

4

100

100

100

5

100

100

100

6

100

100

100

7

100

100

0

 


Animal Weight Loss 

See Weight Loss worksheet on attached Excel file for data.

  Attached Files  
   
 IM004 workbook(10-13-10)-Weight loss, viral titers.xlsx
 image004.png


Lung Viral Load 

See viral titers worksheet in attached Excel file for data.

  Attached Files  
   
 image002.png
 IM004 workbook(10-13-10)-Weight loss, viral titers.xlsx


Viral RNA ( 10e4 PFU dose only) 

These graphs indicate the relative values of either viral genomic RNA or viral mRNA.  Values for each replicate is plotted and the data is available on the attached Excel file.

  Attached Files  
   
 IM004_viral qPCR_analysis.xlsx
 image006.png
 image004.png


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