Determine the effects of knockout of VN1203/SARS common hubs and bottlenecks (from Study 1) on VN1203 pathogenesis.
Use 6 week-old IDO1 KO (bottleneck), TNFRSF1B (hub) KO or WT C57BL/6 mice
- Infect with A/VN1203/04
- MLD50 group: 0.1, 1, 10 and 100 PFU (three mice per group). Mouse body weights and survival were assessed over a 14 day period.
- Lung tissue harvest group: Mock or 1000 PFU, 3 mice per group per day, collect tissues for virus titer and RNA harvest on days 2 and 6. Body weights were also collected each day. This part of the experiment is described in the attached design template.
Attached is a Powerpoint presentation summarizing the viral titers, survival and weight loss results.
Metadata is available in the sub-folders on the right.
Microarray data is also available.