Experiment Overview 

Purpose:
To obtain samples for transcriptional analysis and proteomics in triplicate using wild type virus (icSARS-CoV) and icSARS delta ORF6 in 2B4 cells/sorted Calu3 cells with high ACE2 expression.

Overview of Experiment:
Time Points = 0, 3, 7, 12, 24, 30, 36, 48, 54, 60, and 72 hrs post infection.
Done in triplicate for both RNA and Protein
Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates.
Time matched mocks done in triplicate from same cell stock as rest of samples.
Culture medium (the same as what the virus stock is in) will be used for the mock infections.

 

Metadata is available in the sub-folders on the right.

Microarray data and global proteomics data is also available.


Viral Growth Curves 

Data is attached.

  Attached Files  
   
 SCL005_titers(8-24-10).xls
 image002.png


Viral RNA (qPCR) 

 

Data is attached.

  Attached Files  
   
 SCL005 Viral qPCR Analysis.xlsx
 image002.png
 image004.png
 image006.png


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