Purpose: To obtain samples for transcriptional analysis and proteomics in triplicate using wild type virus and Bat SRBD (spike receptor binding domain from the wild type strain Urbani to allow for infection of human and non-human primate cells) in 2B4 cells/sorted Calu3 cells with high ACE2 expression.
Overview of Experiment:
Time Points = 0, 3, 7, 12, 24, 30, 36, 48, 54, 60, and 72 hrs post infection.
Done in triplicate for both RNA and Protein
Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates.
Time matched mocks done in triplicate from same cell stock as rest of samples.
Culture medium (the same as what the virus stock is in) will be used for the mock infections.
Metadata is available in the sub-folders on the right.
Microarray data and global proteomics data is also available.