Study Design 

Purpose: To analysis the host response at differing viral doses over several days post infection.

General Design:

  • Used 20 week-old C57BL/6 mice
  • Mouse adapted strain of SARS-CoV, MA15.
  • Four Doses = 10^2, 10^3, 10^4, and 10^5 PFU
  • Time points of 1, 2, 4, and 7 days post infection
  • 5 mice/time point for infections
  • 3 mice/time point for time matched mocks
  • Lung tissue was harvested for both RNA and Protein analysis
    • Protein samples from this experiment will be used to build the AMT tag database used in the quantitative proteomic analysis.
  • Determine viral infection phenotypes - % weight loss, survival and viral load in the lung tissue

Metadata is available in the sub-folders on the right.

Microarray data and global proteomics data is also available.