To test our hypotheses that major components of the extracellular matrix remodeling pathways are important for SARS-CoV and Influenza pathogenesis in mice.
Overview of Experiment:
Groups of 20 week old C57BL/6 or PLAT knockout mice will be infected with 10^5 PFU of MA15 or mock infected.
This is a 1 log higher dose than was previously tested in these mice.
Mice will be euthanized on days 4 and 7 post infection to measure virus load and isolate samples for measurement of virus load, lung pathology,transcriptional and proteomics analysis.
Mock infected animals will also be harvested at each timepoint.
Mice will be weighed every 24 hours to measure general disease progression and any mouse approaching 30% weight loss will be euthanized.
Weight loss and survival data is available in the sub-folder on the right.
Microarray data is also available.