To investigate differences between the wild type MA15 and nsp16 mutant virus.
Overview of Experiment:
Groups of 20 week old C57BL6 mice will be infected MA15 or nsp16 at a dose of 10^5 PFU or mock infected.
Mice will be euthanized on 1, 2, 4, and 7 days post-infection to measure virus load and isolate samples for measurement of virus load, lung pathology, transcriptional and proteomics analysis.
Mock infected animals will also be harvested at each time point.
Mice will be weighed every 24 hours to measure general disease progression and any mouse approaching 30% weight loss will be euthanized.
The decision was made to only do 4 replicates of the infected samples and 3 from the time matched mock. Here are the samples that were used for the arrays.
MA15 d1 replicates 1-4; MA15 d2 replicates 1-4; MA15 d4 replicates 1-4; MA15 d7 replicates 1, 2, 4, 5; nsp16 d2 replicates 1-4; nsp16 d4 replicates 1-4; nsp16 d7 replicates 1-4; mock d1 replicates 1-3; mock d2 replicates 1-3; mock d4 replicates 1-3; mock d7 replicates 1-3
Weight Loss and Viral RNA level are available in the sub-folders on the right.
Microarray data is also available.