Study Design 

Purpose: To investigate differences between wild type MA15 infection of C57BL6/J mice and selected knockout mice.

Experimental Design:

  • Groups of 10 week old C57BL/6 mice, IRF3, IRF3/7 or Tnfrsf1a/1b knockout mice will be infected with SARS MA15 at a dose of 10^5 PFU or mock infected.
  • Mice will be euthanized on days 4 and 7 post infection to measure virus load and isolate samples for measurement of virus load, lung pathology,  transcriptional and proteomics analysis.  Mock infected animals will also be harvested at each timepoint.
  • Mice will be weighed every 24 hours to measure general disease progression and any mouse approaching 30% weight loss will be euthanized.
  • Arrays were only done on samples from Tnfrsf1a/1b knockout mice

Weight Loss is available in the sub-folders on the right.

Microarray data is also available.