Purpose: To investigate differences between wild type MA15 infection of C57BL6/J mice and selected knockout mice.
- Groups of 10 week old C57BL/6 mice, IRF3, IRF3/7 or Tnfrsf1a/1b knockout mice will be infected with SARS MA15 at a dose of 10^5 PFU or mock infected.
- Mice will be euthanized on days 4 and 7 post infection to measure virus load and isolate samples for measurement of virus load, lung pathology, transcriptional and proteomics analysis. Mock infected animals will also be harvested at each timepoint.
- Mice will be weighed every 24 hours to measure general disease progression and any mouse approaching 30% weight loss will be euthanized.
Arrays were only done on samples from Tnfrsf1a/1b knockout mice
Weight Loss is available in the sub-folders on the right.
Microarray data is also available.