Proteomics Analysis 

NIAID Systems Virology Center (systemsvirology.org)
We welcome collaborations. When possible please notify us when our data is utilized in publications (honey@uw.edu).
Please acknowledge the Systems Virology Center, NIAID Contract No. HHSN272200800060C when using these data.

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Attached is an Excel spreadsheet ICL006_All Protein Data_Portal.xlsx, which contains the rolled up protein-level data in normalized log base 10 format and unscaled, unlog transformed intensity format for all replicate samples.  If a sample is missing, that means it was eliminated during the QC process.  Statistical significance was determined through G-test (qualitative) and T-test (quantitative) analysis of CA/04 H1N1 2009 versus mock at each time point.  The Summary tab provides detailed information about each spreadsheet.

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Also attached is an excel spreadsheet ICL006_All PEPTIDE Data_Portal.xlsx, which contains the normalized peptide intensity data for all biological replicates and technical replicates that passed QC.  The data are log base 10 transformed and scaled by dividing by 5 x 10^7 (during the peptide matching process, not the statistical process).

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Attached is a Word document ICL006_StatisticsReport_Portal.docx outlining our method for proteomic data processing.

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  Attached Files  
   
 ICL006_All PEPTIDE Data_Portal.xlsx
 ICL006_All Protein Data_Portal.xlsx
 ICL006_StatisticsReport_Portal.docx