Proteomics Analysis 

Attached is an Excel spreadsheet SCL005_Peptide data.xls with the normalized intensity values for all peptides that passed the filtering criteria.  Data are log base 10 intensity values, averages of technical replicates, and provided as biological replicates.  Missing values are indicated as NaN, meaning that no data were present for either technical replicate.

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Attached is an Excel spreadsheet SCL005_Protein data.xls with protein values rolled up from peptide values that contained sufficient information for roll-up.  Data are log base 10 intensity values, and provided as biological replicates.  Missing values are indicated as NaN, meaning that no data were present for any peptide for that protein for that sample.  One column indicates the number of peptides used for the protein value.

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Attached is an Excel spreadsheet SCL005_Protein_data_unscaled, which contains the entire protein dataset unscaled and unlog transformed, in addition to log base 2 and log base 10 transformations.

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Attached is an Excel spreadsheet SCL005_Sig Protein list.xlsx, which contains all p-values for quantitative (ANOVA) and qualitative (G-test) assessment of significance.  There are two worksheets: one for significant at any time point and a second for those proteins that are not significant by any comparison.  The p-values are listed as: 0 (non-significant), 1 (significant-up regulated), and -1 (significant-down regulated).  Log base 10 ratios of each time point to its time-matched mock are also included and colored for red=up and green=down and by >2-fold change (log 10 = 0.3).  Comparison of WT to DORF6 at each time point has also been included.

  Attached Files  
   
 SCL005_Peptide data.xls
 SCL005_Protein data.xls
 SCL005_Sig Protein list.xlsx
 SCL005_Protein_data_unscaled.xlsx