To obtain samples for transcriptional analysis and proteomics in triplicate using the VN1203 pathogenicity mutants PB1-F2del and PB2-627E. (There are a two time points for wild type VN1203)
Overview of Experiment:
Time Points = 0, 3, 7, 12, 18, and 24 hrs post infection.
Done in triplicate for both RNA and Protein
Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates.
Time matched mocks done in triplicate from same cell stock as rest of samples.
Culture medium (the same as what the virus stock is in) will be used for the mock infections.
Metadata is available in the sub folders on the right.
Microarray data and global proteomics data is also available.